(PDF) Separation of CHO cells using hydrocyclones
(55) Mammalian cell/perfusion cultures Small The perfusion cultivation could be performed maintaining high constant separation efficiency and high cell viability by using the small hydrocyclone. ...
Use of Hydrocyclones for Mammalian Cell …
A hydrocyclone with a volume of 2.56 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply).
Application of Hydrocyclone for Cell Separation in ...
Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte Dissertation von Elsayed Ahmed Elsayed Ahmed
hydrocyclone mammalian cells - palestra …
MEETING ABSTRACT Open Access Application of , - Hydrocyclone can be successfully used for cell separa- tion in continuous mammalian perfusion bioreactors - Cells adapt themselves to the shear forces inside the
Application of Hydrocyclone for Cell Separation in ...
Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures. Göttingen : Cuvillier Verlag, ©2005: Material Type: Document, Internet resource: Document Type: Internet Resource, Computer File: All Authors / Contributors: Elsayed Ahmed Elsayed Ahmed
The Potential of Hydrocyclone Application for …
DOI: 10.6000/1927-3037.2013.02.04.2 Corpus ID: 84350518. The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors @inproceedings{Elsayed2013ThePO, title={The Potential of Hydrocyclone Application for Mammalian Cell Separation in Perfusion Cultivation Bioreactors}, author={Elsayed A Elsayed …
Data Hydrocyclone SBL2001-e - …
hydrocyclone mammalian cell retention device for single-use and reusable bioreactors perfusion technologies overflow (harvest) feed feed product specification. Anmelden Registrieren; Verstecken. Data Hydrocyclone SBL2001-e. Universität. Universität Potsdam. Kurs. Literaturwissenschaft 1 FRZVL1. Hochgeladen von.
The Use of Hydrocyclones for Mammalian Cell …
A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as 99%. Due to the fact that a 5-L-bioreactor was used, a sub-optimal configuration of overflow and underflow diameters was used in order to obtain high cell viabilities combined with separation efficiencies suitable for cell …
Application of hydrocyclone for cell separation in ...
Application of hydrocyclone for cell separation in mammalian cell perfusion cultures [Elektronische Ressource] / von Elsayed Ahmed Elsayed Ahmed : Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität Carolo-Wilhelmina zu …
Application of Hydrocyclone for Cell Separation in ...
Moreover, operating the hydrocyclone at pressures ranging from 0.85 to 1.40 bar is favorable, since the hydrocyclone performance is expected to be constant in this range. Due to the high flow rates during hydrocyclone operation, and to avoid the complete washout of bioreactor content, the hydrocyclone was intermittently operated during the experimental work.
Use of Hydrocyclones for Mammalian Cell ... - …
A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply). Cultures …
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To begin, grow between 2 x 107 and 2.5 x 107 mammalian cells, either adherent or in suspension, and crosslink the cells. jove.com Om te beginnen, groeien tussen de 2 x 10 7 en 2,5 x 10 7 cellen van zoogdieren, ofwel aanhanger of in suspensie, en verknopen van de cellen .
Application of Hydrocyclone for Cell Separation in ...
In the present work, the application of a hydrocyclone for cell separation in continuously perfused cultures of mammalian cells was investigated. The characterization of the hydrocyclone performance showed, that a pressure range of 0.85 to 1.40 bar is most suitable to achieve a reliable and stable separation process. Secondly, the effect of hydrocyclone operation on the cell …
Application of Hydrocyclone for Cell Separation in ...
The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures. Um Ihnen ein besseres Nutzererlebnis zu bieten, verwenden wir Cookies.
Application of Hydrocyclone for Cell Separation in ...
Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures (English shop) Elsayed Ahmed Elsayed Ahmed (Author) Preview. Table of Contents, Datei (88 KB) ... The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures.
Application of Hydrocyclone for Cell Separation in ...
Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures. Elsayed Ahmed Elsayed Ahmed. Cuvillier Verlag, 2005 - 140 pages. 0 Reviews .
Cell culture - Wikipedia
Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or medium that supplies ...
Lentiviral transduction of mammalian cells for fast ...
20-4-2020 · 1. Nat Protoc. 2018 Dec;13(12):2991-3017. doi: 10.1038/s41596-018-0075-9. Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins.
Application of Hydrocyclone for Cell Separation in ...
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Application of hydrocyclones for continuous …
- Hydrocyclone can be successfully used for cell separation in continuous mammalian perfusion bioreactors. - Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. - Higher separation efficiencies up to 96% can be achieved depending on the operating HC pressure.